Sigma′s GenElute Complete RNA Purification Equipment supplies a speedy technique for the isolation and purification of whole RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package purifies all sizes of RNA, from giant mRNA and ribosomal RNA all the way down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from different mobile parts reminiscent of proteins, with out using phenol or chloroform. The purified RNA is of the very best integrity, and can be utilized in quite a few downstream purposes together with actual time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.
Isolate true whole RNA together with siRNA and microRNA
Quickly take away contaminating genomic DNA with out using enzymes
No phenol or chloroform extractions
Purify high-quality RNA in 20 minutes
Extract RNA from as little as a single cell
Isolate from all kinds of specimens
The purification strategies of our Complete RNA Purification Kits vary from natural extraction reagents all the best way to spin columns and magnetic beads.
Beginning with top quality, pure, and intact whole RNA is important to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk under will show you how to select the precise Invitrogen product to purify whole RNA out of your particular pattern kind and pattern measurement.
Thermo Scientific GeneJET RNA Purification Equipment is an easy and environment friendly system for purification of whole RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.
The package makes use of a silica-based membrane expertise within the type of a handy spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides will be remoted with the GeneJET RNA Purification Equipment in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in a variety of downstream purposes, together with RT-PCR, RT-qPCR, Northern blotting, and different RNA-based analyses.
RNA purification
Highlights
• Common – can be utilized for each cell and tissue samples from a variety of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and micro organism • Environment friendly – excessive yields of whole RNA. • Quick – 15 minutes after lysis step • Pure – an A260/280 ratio > 1.9 • Handy – spin columns are capped and assembled with assortment tubes
Purposes
RNA purification package
• Quick extraction of excessive purity RNA appropriate for all standard molecular biology procedures, together with: • RT-PCR • RT-qPCR • Northern blotting • Nuclease safety assayIncludes Proteinase Ok Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol
Software
GenElute Complete RNA Purification Equipment is used for the extraction of whole RNA:
From complete blood samples within the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
From an in a single day tradition of bacterial strains in TSB medium in figuring out the consequences of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
GenElute™ Complete RNA Purification Equipment has been used to:
Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
RT-PCR for each giant mRNA and small RNA together with miRNA
Expression profiling
Subsequent Technology Sequencing for RNA and miRNA
