Using Brain Homogenate For Elisa Assay

Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

Description: MTT Cell Proliferation and Viability Assay Kit

Description: The TIGIT:CD112 Homogeneous Assay Kit is designed to measure the_x000D_inhibition of human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) binding to CD112_x000D_(PVRL2/Nectin-2). The TIGIT:CD112 Homogeneous Assay Kit comes in a convenient_x000D_AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD112, and assay buffer to_x000D_perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are_x000D_required. First, a sample containing TIGIT and an inhibitor of choice is incubated with the_x000D_CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading_x000D_the Alpha-counts.

Description: The UTX Homogeneous Assay Kit is designed to measure the activity of UTX for screening and profiling applications. The key to the UTX Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, UTX enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The LSD1 Homogeneous Assay Kit is designed to measure activity of_x000D_lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Homogeneous_x000D_Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this_x000D_kit, only three simple steps on a microtiter plate are required for demethylase detection._x000D_First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next,_x000D_acceptor beads and primary antibody are added, then donor beads, followed by reading_x000D_the Alpha-counts.

Description: The PHF8 Homogeneous Assay Kit is designed to measure PHF8activity for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is aJumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylationactivity toward H3-K9Me2. The PHF8 Homogeneous Assay Kit comes in a convenientAlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primaryantibody, demethylase assay buffer, and purified PHF8 for 96 enzyme reactions. The key to thePHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylatedsubstrate. With this kit, only three simple steps on a microtiter plate are required formethyltransferase detection. First, a sample containing PHF8 enzyme is incubated with thebiotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads,followed by reading the Alpha-counts._x000D_

Description: The PHF8 Homogeneous Assay Kit is designed to measure the activity of_x000D_PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is_x000D_a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation_x000D_activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a_x000D_convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody,_x000D_demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8_x000D_Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate._x000D_With this kit, only three simple steps on a microtiter plate are required for demethylase activity_x000D_detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate._x000D_Next, acceptor beads and primary antibody are added, then donor beads, followed by reading_x000D_the Alpha-counts.

Description: The CD226:CD112 Homogeneous Assay Kit is designed to measure the_x000D_inhibition of CD226 binding to CD112 (PVRL2). The CD226:CD112 Homogeneous Assay Kit_x000D_comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD112,_x000D_and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a_x000D_microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is_x000D_incubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads,_x000D_followed by reading the Alpha-counts.

Description: The CD226:CD155 Homogeneous Assay Kit is designed to measure the_x000D_inhibition of CD226 binding to CD155 (PVR). The CD226:CD155 Homogeneous Assay Kit_x000D_comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD155,_x000D_and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a_x000D_microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is_x000D_incubated with the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads,_x000D_followed by reading the Alpha-counts.

Description: The JMJD3 Homogeneous Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3, also known as KDM6B, is a JumonjiC (JmjC) domain protein that exhibits demethylation activity toward H3-K₂₇Me³ and H3-K₂₇Me². The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JMJD3 Homogeneous Assay Kit is designed to measure activity of the JMJD3 for screening and profiling applications, using AlphaLISA® technology. The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads,_x000D_followed by reading the Alpha-counts.

Description: The Homogeneous Caspase-3 Assay Kit is a complete assay system designed to measure Caspase-3 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Description: The Homogeneous Caspase-7 Assay Kit is a complete assay system designed to measure Caspase-7 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-7 activity measurements. In addition, the kit includes purified Caspase-7 enzyme and a potent Caspase-3/7 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-7 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-7 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Description: The Homogeneous Caspase-6 Assay Kit is a complete assay system designed to measure Caspase-6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-6 activity measurements. In addition, the kit includes purified Caspase-6 enzyme and a potent Caspase-6 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-6 activity level. The fluorogenic substrate, Ac-VEID-AFC, is incubated with purified Caspase-6 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Description: The Homogeneous Caspase-8 Assay Kit is a complete assay system designed to measure Caspase-8 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-8 activity measurements. In addition, the kit includes purified Caspase-8 enzyme and a potent Caspase-8 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-8 activity level. The fluorogenic substrate, Ac-IETD-AFC, is incubated with purified Caspase-8 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Description: The 3x BRD Homogeneous Assay Buffer 1 is optimized for use with the following BPS Bioscience assay kits:
• BRD2 (BD2) Inhibitor Screening Assay Kit #32522 384 reactions
• BRD3 (BD1) Inhibitor Screening Assay Kit #32513 384 reactions
• BRD3 (BD2) Inhibitor Screening Assay Kit #32523 384 reactions
• BRD4 (BD1) Inhibitor Screening Assay Kit #32514 384 reactions
• BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions_x000D_NOTE: This product was formerly known as 3x BRD Assay Buffer.

Description: The 3x BRD Homogeneous Assay Buffer 2 is optimized for use with the following BPS Bioscience assay kits:
• ATAD2A Inhibitor Screening Assay Kit #32601 384 reactions
• ATAD2B Inhibitor Screening Assay Kit #32605 384 reactions
• BRD1 Inhibitor Screening Assay Kit #32521 384 reactions
• BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions
• BRD9 (BD1) Inhibitor Screening Assay Kit #32519 384 reactions
• BRPF3 Inhibitor Screening Assay Kit #32608 384 reactions
• CECR2 Inhibitor Screening Assay Kit #32611 384 reactions
• CREBBP Inhibitor Screening Assay Kit #32607 384 reactions
• SMARCA2 Inhibitor Screening Assay Kit #32610 384 reactions
• SMARCA4/BRG1 Inhibitor Screening Assay Kit #32609 384 reactions
• TAF1 (BD1/BD2) Inhibitor Screening Assay Kit #32604 384 reactions
• TAF1L (BD2) Inhibitor Screening Assay Kit #32602 384 reactions
• TAF1L (BD1/BD2) Inhibitor Screening Assay Kit #32603 384 reactions
• TRIM24 Inhibitor Screening Assay Kit #32606 384 reactions_x000D_NOTE: This product was formerly named 3x ATAD2 Assay Buffer.

Description: The 3x BRD Homogeneous Assay Buffer 3 is optimized for use with the following BPS Bioscience assay kits:_x000D_• BAZ2B Inhibitor Screening Assay Kit #32600 384 reactions_x000D_NOTE: This product was formerly known as BAZ2B Assay Buffer.

Description: The JMJD1A Homogeneous Assay Kit is designed to measure activity of the JMJD1A for_x000D_screening and profiling applications. JMJD1A, also known as KDM3A, is a JumonjiC (JmjC)_x000D_domain-containing histone lysine demethylase that exhibits demethylation activity toward monoand_x000D_dimethyl-lysine 9 on histone H3. The key to the_x000D_JMJD1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated_x000D_substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase_x000D_activity detection. First, a sample containing JMJD1A enzyme is incubated with the biotinylated_x000D_substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by_x000D_reading the Alpha-counts._x000D_