Using Brain Homogenate For Elisa Assay

Description: The PRMT4 Homogeneous Assay Kit is designed to measure PRMT4 activity for screening and profiling applications. PRMT4 (CARM1) is an arginine methyltransferase and dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3. The PRMT4 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT4 substrate, primary antibody, methylation assay buffer, and purified PRMT4 for 384 enzyme reactions. The key to the PRMT4 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT4 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The PRMT9 Homogeneous Assay Kit is designed to measure PRMT9 activity for screening and profiling applications. PRMT9 is a histone methyltransferase that exhibits methylation activity toward an arginine residue of SAP145. The PRMT9 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with GST-tagged PRMT9 substrate, primary antibody, methylation assay buffer, and purified PRMT9 for 384 enzyme reactions. _x000D_ The key to the PRMT9 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT9 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JMJD1A Homogeneous Assay Kit is designed to measure JMJD1A activity for screening and profiling applications. JMJD1A, also known as JHDM2A and KDM3A, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K9Me2 and H3-K9Me1. The key to the JMJD1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts._x000D_ Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JMJD2B Homogeneous Assay Kit is designed to measure JMJD2B activity for screening and profiling applications. JMJD2B, also known as JHDM3B and KDM4B, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3. The key to the JMJD2B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts._x000D_

Description: The JHDM1D Homogeneous Assay Kit is designed to measure the activity of the JHDM1D for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JHDM1D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JMJD2D Homogeneous Assay Kit is designed to measure JMJD2D activity for screening and profiling applications. JMJD2D, also known as KDM4D, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me³ and H3-K₃₆Me³. The key to the JMJD2D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts._x000D_ Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above.

Description: The JMJD1B Homogeneous Assay Kit is designed to measure JMJD1B activity for screening and profiling applications. JMJD1B, also known as JHDM2B and KDM3B, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me³ and H3-K₃₆Me³. TThe key to the JMJD1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts._x000D_Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above.

Description: The TIGIT:CD155 Homogeneous Assay Kit is designed to measure the_x000D_inhibition of TIGIT binding to CD155. The TIGIT:CD155 Homogeneous Assay Kit comes in a_x000D_convenient AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD155, and assay_x000D_buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter_x000D_plate are required. First, a sample containing TIGIT and an inhibitor of choice is incubated with_x000D_the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by_x000D_reading the Alpha-counts.

Description: The TIGIT:CD112 Homogeneous Assay Kit is designed to measure the_x000D_inhibition of human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) binding to CD112_x000D_(PVRL2/Nectin-2). The TIGIT:CD112 Homogeneous Assay Kit comes in a convenient_x000D_AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD112, and assay buffer to_x000D_perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are_x000D_required. First, a sample containing TIGIT and an inhibitor of choice is incubated with the_x000D_CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading_x000D_the Alpha-counts.

Description: The UTX Homogeneous Assay Kit is designed to measure the activity of UTX for screening and profiling applications. The key to the UTX Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, UTX enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The LSD1 Homogeneous Assay Kit is designed to measure activity of_x000D_lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Homogeneous_x000D_Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this_x000D_kit, only three simple steps on a microtiter plate are required for demethylase detection._x000D_First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next,_x000D_acceptor beads and primary antibody are added, then donor beads, followed by reading_x000D_the Alpha-counts.

Description: The PHF8 Homogeneous Assay Kit is designed to measure PHF8activity for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is aJumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylationactivity toward H3-K9Me2. The PHF8 Homogeneous Assay Kit comes in a convenientAlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primaryantibody, demethylase assay buffer, and purified PHF8 for 96 enzyme reactions. The key to thePHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylatedsubstrate. With this kit, only three simple steps on a microtiter plate are required formethyltransferase detection. First, a sample containing PHF8 enzyme is incubated with thebiotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads,followed by reading the Alpha-counts._x000D_

Description: The PHF8 Homogeneous Assay Kit is designed to measure the activity of_x000D_PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is_x000D_a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation_x000D_activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a_x000D_convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody,_x000D_demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8_x000D_Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate._x000D_With this kit, only three simple steps on a microtiter plate are required for demethylase activity_x000D_detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate._x000D_Next, acceptor beads and primary antibody are added, then donor beads, followed by reading_x000D_the Alpha-counts.

Description: The JMJD3 Homogeneous Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3, also known as KDM6B, is a JumonjiC (JmjC) domain protein that exhibits demethylation activity toward H3-K₂₇Me³ and H3-K₂₇Me². The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JMJD3 Homogeneous Assay Kit is designed to measure activity of the JMJD3 for screening and profiling applications, using AlphaLISA® technology. The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads,_x000D_followed by reading the Alpha-counts.

Description: The Homogeneous Caspase-7 Assay Kit is a complete assay system designed to measure Caspase-7 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-7 activity measurements. In addition, the kit includes purified Caspase-7 enzyme and a potent Caspase-3/7 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-7 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-7 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Description: The CD226:CD112 Homogeneous Assay Kit is designed to measure the_x000D_inhibition of CD226 binding to CD112 (PVRL2). The CD226:CD112 Homogeneous Assay Kit_x000D_comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD112,_x000D_and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a_x000D_microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is_x000D_incubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads,_x000D_followed by reading the Alpha-counts.