Western Blot Stripping Buffer
The Western BLoT Stripping Buffer is an answer for eradicating major and secondary antibodies from probed Western blot membranes. Antibody removing with this buffer can happen below delicate situations (room temperature, 30 min incubation), minimizing lack of immobilized protein from the membrane. When utilizing a PVDF membrane, the identical membrane could be stripped and reprobed 2–5 instances. After stripping, membranes could be re-probed, both with a special focus of major antibody or with a completely totally different major antibody.
Cat. # | Product | Measurement | License | Amount | Particulars | |
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T7135A | Western BLoT Stripping Buffer | 500 mL | * | |||
The Western BLoT Stripping Buffer is a reagent that may take away major and secondary antibodies from Western blot membranes. After remedy with the Stripping Buffer, the membrane could be reused; it’s attainable to probe the membrane with both a special focus of major antibody or with a completely totally different major antibody. With this product, the antibody removing response proceeds below comparatively delicate situations (room temperature, 30 minutes), and due to this fact there may be little or no lack of immobilized protein from the membrane. When utilizing a PVDF membrane, the identical membrane could be stripped and re-probed 2–5 instances. |
Title: Antibody Stripping Resolution
Catalog quantity: L7710A, 500ml* GV0480, 500ml 5X
*Adequate reagents for 3000 cm2 or 200-400 membrane strips or 20-40 commonplace blots
• Storage: Retailer at RT ( for a protracted storage: +4C°) (L)
● No pungent smelling mercaptoethanol Save time
● Antibody stripping is completed at room temperature. No heating of blots required Saves expensive pattern
● Strip antibodies in nearly 15 minutes at room temperature Economical
● Reblocking of blots could also be prevented in most situations

Blocking buffer | Blocking agent | Highlights | When to make use of | Out there codecs |
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StartingBlock |
Serum and biotin-free single purified protein |
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PBS TBS PBST TBST |
Blocker FL |
Single purified protein |
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10X |
Pierce Clear Milk Blocking Buffer |
Clarified and stabilized milk proteins |
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Borate, pH 7.6 |
SuperBlock |
Serum and biotin-free single purified glycoprotein |
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PBS TBS PBST TBST |
SEA BLOCK Blocking Buffer |
Steelhead salmon serum |
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PBS |
Blocker BSA |
Purified bovine serum albumin |
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PBS TBS |
Blocker Casein |
Purified casein |
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PBS TBS |
Blocker BLOTTO |
Non-fat dry milk |
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TBS |
Protein-Free |
Non-protein blocking compound |
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PBS TBS PBST TBST |
Pierce Quick Blocking Buffer |
Single purified protein |
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TBS |
SuperSignal Western Blot Enhancer |
Membrane remedy for low abundance or poor immunoreactivity antibodies |
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Prepared-to-use |
Stripping and re-probing of Western blots gives a number of benefits:
- Conservation of samples which might be costly or obtainable solely in restricted portions;
- Evaluation of a given blot utilizing a number of totally different antibodies, e.g. subtype- or isoform-specific antibodies;
- Re-analysis of anomalous outcomes and affirmation with the identical or a special antibody;
- Correcting errors in incubation with the incorrect antibody;
- Price financial savings in reagents and time by reusing the identical blot.
A number of protocols for antibody stripping from Western blots have been printed, together with these which make the most of low pH, warmth and detergent, and chaotropic brokers. Three advisable protocols are offered under. The primary is relevant to any chemiluminescent substrate system and makes use of a mixture of detergent and warmth to launch the antibodies. The second is often used for functions the place antibodies need to be separated from an antigen and employs low pH to change the construction of the antibody in such a means that the binding web site is now not lively.
The third protocol employs the ReBlot™ Plus Western Blot Recycling Package which has been particularly formulated for stripping antibodies from Western blot membranes. Advantages of this strategy embody
- Avoidance of odor associated to β-mercaptoethanol.
- Room temperature processing.
- Elimination of antibodies in 15 minutes.
None of those strategies will take away the coloured precipitates generated from chromogenic detection techniques (e.g., BCIP, 4CN, DAB and TMB). Nevertheless, it’s nonetheless attainable to research the blot with one other antibody particular to a special goal protein.
Often, these protocols ought to solely be used for qualitative functions, except it has been established that stripping doesn’t quantitatively have an effect on a given antigen. Relying upon the tactic and kind of membrane used, many antigens will stand up to not less than 5 stripping cycles. Nevertheless, it’s to be considered that in every stripping cycle small parts of membrane-immobilized proteins might be eliminated. When a number of antigens are to be detected sequentially, it is strongly recommended to begin with antigens that are anticipated to happen at decrease abundance or yield much less sign.
Listed below are some extra suggestions when planning a Western blot experiment with a number of rounds of antibody stripping.
- PVDF membranes are extra strong than nitrocellulose and are due to this fact advisable for any protocol involving antibody stripping.
- Drying of PVDF membranes instantly after switch from the SDS-PAGE gel improves binding of proteins to the membrane and is especially advisable when a number of stripping is deliberate. Dry PVDF membranes should be rewetted with alcohol previous to the primary spherical of immunodetection.
- Detect low-abundance antigens first.
- Use low-affinity antibodies earlier than high-affinity antibodies.
- Necessary: Though drying of a PVDF blot is advisable instantly after switch, the blot shouldn’t be allowed to dry between rounds of immunodetection. Any residual antibody molecules will bind completely to the membrane whether it is allowed to dry.
West Ez Stripping Buffer, Economic Size |
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S2100-100 | GenDepot | 2x500ml | EUR 336 |
Western blot recycling stripping buffer, 50 ml (10X) |
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90101 | Alpha Diagnostics | 50 ml | EUR 285.6 |
Phospho Antibody Stripping Solution |
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AKR-102 | Cell Biolabs | 1 kit | EUR 470.4 |
Description: This reagent allows you to strip phospho antibodies from protein blots and subsequently re-probe the same blot. |
101Bio WB Stripping Solution |
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P5W3 | 101Bio | - | Ask for price |
Dura Mater Stripping Forcep Angled |
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INS6134 | Scientific Laboratory Supplies | EACH | EUR 112.8 |
Dura Mater Stripping Forcep Straight |
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INS6132 | Scientific Laboratory Supplies | EACH | EUR 112.8 |
T-Pro Western Blot Stripping Reagent |
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JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 213.6 |
Human IgG-RF stripper/Adsorbent (1 ml, sufficient for stripping 50 samples of 100 ul human serum/plasma) |
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RFS-1 | Alpha Diagnostics | 1 ml | EUR 123.6 |
100ML ProtoLift Western Strip Buffer |
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NAT1256 | Scientific Laboratory Supplies | 100ML | EUR 92.7 |
PhastGel SDS Buffer Strips pack of 10 |
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17051601 | Scientific Laboratory Supplies | EACH | EUR 116.28 |
PhastGel Native Buffer Strips pack of 10 |
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17051701 | Scientific Laboratory Supplies | EACH | EUR 116.28 |
Antibody diluent buffer |
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AR1106-2 | BosterBio | 12ml | EUR 109.2 |
ExcelGel SDS Buffer Strips anode and cathode 6 pairs |
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17134201 | Scientific Laboratory Supplies | EACH | EUR 154.8 |
Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers |
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5730 | Scientific Laboratory Supplies | EACH | EUR 955.2 |
Corning 3D Clear Antibody Buffer - 100mL |
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5735 | Scientific Laboratory Supplies | EACH | EUR 265.2 |
Corning 3D Clear Antibody Buffer - 30mL |
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5734 | Scientific Laboratory Supplies | EACH | EUR 134.4 |
ChonBlock Detection Antibody Dilution Buffer, 100 ml |
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90681 | Chondrex | 100 ml | EUR 155.7 |
Description: ChonBlock Detection Antibody Dilution Buffer |
Antibody Dilution Buffer for ICC and IHC |
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SF40010 | Neuromics | 10 ml | EUR 146.4 |
Western Blot Primary Antibody Diluent Buffer |
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abx090689-100ml | Abbexa | 100 ml | EUR 218.4 |
Citrate Buffer with Tween pH 6.0 (Antigen Retrieval Buffer) |
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RRSP5115-E | Atom Scientific | 1L | EUR 10.83 |
Citrate Buffer with Tween pH 6.0 (Antigen Retrieval Buffer) |
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RRSP5115-F | Atom Scientific | 2.5L | EUR 18.17 |
Borax Buffer 0.00996M |
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BOR0091 | Scientific Laboratory Supplies | 1L | EUR 76.8 |