Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are typically found throughout the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.
Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
Detect glycoconjugates in microscopy and circulation cytometry
Stain glycoproteins in gels
Withstands fixation and permeabilization
Number of 10 CF dyes from UV to near-infrared
Superior CF dyes are good, photostable, and water-soluble
Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric functions and for gel staining of glycoproteins. In neutral and alkaline choices, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic choices (pH beneath 5.0), Con A exists as a dimer. Con A might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles throughout the secretory pathway.
BioMag Plus
DESCRIPTION Utility
Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been moreover used throughout the preparation of protein loaded microgels to cease enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).
Packaging
1 g in poly bottle
25, 100, 250 mg in poly bottle
Biochem/physiol Actions
Con A should not be blood group explicit nevertheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for train. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are long-established. Con A shows mitogenic train which depends on its diploma of aggregation. Succinylation results in an brisk dimeric kind which stays a dimer above pH 5.6.
Preparation Discover
Extraordinarily purified by explicit affinity chromatography.
Analysis Discover
Agglutination train: Determined from serial dilutions of a 1 mg/mL reply (this train is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Sort A Human Blood.
Concanavalin A (Con A) is probably going probably the most typically used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A shows the extreme, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.
Uncover the Correct Stain for Your Utility
Con A and completely different lectins are carbohydrate binding proteins that acknowledge explicit sugar moieties on glycoproteins. The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, completely different cell ground stains or completely different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, may produce larger ground staining and is also further acceptable in your cell type. Lectin conjugates might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles throughout the secretory pathway.
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule
