BrdU Cell Proliferation

BrdU Cell Proliferation ELISA Kit

Key selections and particulars

  • Sensitivity: 40 cells/efficiently
  • Pattern selection: Adherent cells, Suspension cells
  • Detection strategy: Colorimetric
  • Assay selection: Sandwich (qualitative)

5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It will get built-in into the newly synthesized DNA of proliferating cells as an alternative of thymidine. Biovision’s BrdU Cell Proliferation Assay Bundle deal detects built-in BrdU utilizing a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody certain to BrdU, which is adopted by addition of TMB (a HRP substrate). The extent of coloration enchancment is proportional to the amount of BrdU built-in into the cells and could possibly be utilized straight as an indicator of cell proliferation. In contrast with utterly totally different cell proliferation assays, this bundle deal detects solely the proliferating cells and in no way the seeded cells. This terribly delicate, non-radioactive bundle deal detects as so much a lot much less as 50-100 proliferating cells.

Background

Halogenated nucleotides such because of the pyrimidine analog bromodeoxyuridine (BrdU) are helpful for labeling nascent DNA in residing cells and tissues. BrdU turns into built-in into replicating DNA as an alternative of thymidine and subsequent immunodetection of BrdU utilizing particular monoclonal antibodies permits labeling of cells in S part of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb could possibly be utilized to detect BrdU built-in into single stranded DNA.

Product Description

The BrdU Cell Proliferation Assay Bundle deal detects 5-bromo-2’-deoxyuridine (BrdU) built-in into mobile DNA all by way of cell proliferation utilizing an anti-BrdU antibody. When cells are cultured with labeling medium that comes with BrdU, this pyrimidine analog is built-in as an alternative of thymidine into the newly synthesized DNA of proliferating cells.

After eradicating labeling medium, cells are fastened and the DNA is denatured with our fixing/denaturing reply. Then a BrdU mouse mAb is added to detect the built-in BrdU (The denaturing of DNA is crucial to bolster the accessibility of the built-in BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to acknowledge the certain detection antibody. HRP substrate TMB is added to develop coloration. The magnitude of the absorbance for the developed coloration is proportional to the amount of BrdU built-in into cells, which is a direct indication of cell proliferation.

Product Consists of Amount (with Rely) Reply Shade
BrdU 1 x 150 µl
Fixing/denaturing Reply 2 x 25 ml
BrdU Mouse Detection mAb 1 x 500 µl Inexperienced
Anti-mouse IgG, HRP-Linked Antibody 1 x 500 µl Pink
Detection Antibody Diluent 1 x 50 ml Inexperienced
HRP Diluent 1 x 50 ml Pink
20X Wash Buffer 1 x 50 ml
TMB Substrate 7004 1 x 50 ml
STOP Reply 7002 2 x 25 ml

 

BrdU Cell Proliferation ELISA Kit (colorimetric)
BrdU Cell Proliferation ELISA Package (colorimetric)

 

 

Brdu Cell
Brdu Cell

METABOLIC PROLIFERATION ASSAYS

Assays that measure metabolic prepare are acceptable for analyzing proliferation, viability, and cytotoxicity. The low price of tetrazolium salts identical to MTT, XTT, and WST-1 to coloured formazan compounds or the bioreduction of resazurin happens solely in metabolically energetic cells. Actively proliferating cells enhance their metabolic prepare, whereas cells uncovered to toxins may need decreased prepare.

MTT Cell Proliferation Assays

MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide; thiazolyl blue) is a water soluble tetrazolium salt yielding a yellowish reply when ready in media or salt selections missing phenol pink. Dissolved MTT is transformed to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water-insoluble formazan is perhaps solubilized utilizing isopropanol or utterly totally different solvents, and the dissolved provides is measured spectrophotometrically utilizing absorbance as a operate of focus of transformed dye.

XTT Cell Proliferation Assays

In distinction to MTT, the cleavage product of XTT is soluble in water; a solubilization step is subsequently not required. The tetrazolium salt XTT is cleaved to formazan by a elaborate mobile mechanism. This bioreduction happens in viable cells solely, and is expounded to NAD(P)H manufacturing by means of glycolysis. The quantity of formazan dye frequent straight correlates to the variety of metabolically energetic cells contained in the customized.

WST-1 Cell Proliferation Assays

The common tetrazolium salt WST-1 is cleaved to a soluble formazan by a elaborate mobile mechanism that happens completely on the cell flooring. This bioreduction is especially counting on the glycolytic manufacturing of NAD(P)H in viable cells. The quantity of formazan dye frequent straight correlates to the variety of metabolically energetic cells contained in the customized.

BrdU Cell Proliferation

MBS396131-200Tests 200Tests
EUR 415

BrdU Cell Proliferation

MBS396131-5x200Tests 5x200Tests
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BrdU Cell Proliferation Assay Kit

C0300-050 500 Assays
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BrdU Cell Proliferation Assay Kit

C0300-100 1000 Assays
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BrdU Cell Proliferation Assay Kit

C0300-500 5000 Assays
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BrdU Cell Proliferation Assay Kit

K306-1000 each
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BrdU Cell Proliferation Assay Kit

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Chemiluminescent BrdU Cell Proliferation

MBS396130-200Tests 200Tests
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Chemiluminescent BrdU Cell Proliferation

MBS396130-5x200Tests 5x200Tests
EUR 2260

Chemiluminescent BrdU Cell Proliferation

MBS396134-1000Tests 1000Tests
EUR 870

Chemiluminescent BrdU Cell Proliferation

MBS396134-5x1000Tests 5x1000Tests
EUR 4020

BrdU Cell Proliferation Colorimetric Assay

TBS2086 200 tests
EUR 380

CytoSelect™ MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 320

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 320

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4380754-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4380754-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4380754-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4380754-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4380754-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4381463-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4381463-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

Bromodeoxyuridine (BrdU) (Proliferation Marker)

MBS4381463-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

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