Corticosterone ELISA gear is a aggressive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone ranges in plasma, serum, urine, milk, saliva and cell custom supernatant.
A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Necessities or check out samples are added to the wells and subsequently biotinylated Corticosterone is added after which adopted by washing with wash buffer. Avidin-Biotin-Peroxidase Superior is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualise HRP enzymatic response. TMB is catalyzed by HRP to offer a blue coloration product that modifications into yellow after together with acidic stop decision. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.
corticosterone
Product Particulars
Sensitivity:
27.Zero pg/ml (differ 32 – 20,000 pg/ml)
Assay Time:
Three hours
Functions:
ELISA, Colorimetric detection
Software program Notes:
For the quantitative willpower of Corticosterone in custom supernatants, feces, plasma, serum, and saliva from any species. Cited sample kinds embrace cell lysate, urine and full blood.
Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone manufacturing has been confirmed to increase with stress, with elevated corticosterone ranges being associated to impairment of future memory retrieval and following burn injury. Together with stress ranges, corticosterone is believed to play a decisive operate in sleep-wake patterns.
Immediate Protocol for Small Volumes of Serum/Plasma Samples
Let all choices come to room temp sooner than use.
Aliquot 10µl of each sample into microfuge tube.
Make 1ml 1:100 Steroid Displacement Reagent (SDR) decision (immediately sooner than use) in deionized water or PBS (not assay buffer)
Add 10µl 1:100 SDR to each sample tube.
Vortex, let stand >5 minutes sooner than diluting with EIA buffer.
Add 380µl EIA assay buffer to each plasma tube, vortex.
Final dilution is ~1:40 (This remaining dilution is not going to be acceptable for every sample; thus, optimum dilution must be determined by each end shopper for his or her experimental sample group.)
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