Innovex Fc Receptor Blocker is a UNIVERSAL Fc Blocker related to blocking all types of Fc receptors equal to Fc-gamma receptors of type I,II and III; Fc -epsilon receptors type I and II; Fc –alpha receptors, Fcα/ µR and FcRn. Innovex Fc Blocker does NOT comprise antibodies, Immunoglobulins or immunoglobulin fragments. INNOVEX Fc Blocker is perhaps universally utilized to dam all types of Fc receptors in all-species along with human, mouse and all-animals This Widespread Fc Block could be utilized in a variety of Immunoassays equal to IHC, ICC, Immunofluorescence and Circulation cytometry. INNOVEX UNIVERSAL Fc BLOCK is suitable for blocking Fc receptors in frozen and paraffin tissuesections and cyto preps of human and all-animal species. INNOVEX Fc Blocker may also be typically used for eliminating background staining in thoughts cells/tissues.
innovexbio common fc receptor blocker
INTRODUCTION
Fc receptors (FcRs) are glycoproteins of approximate molecular weights of 50-70 kD. They’re extensively expressed
all by means of the immune cells. Fc receptors are present on leukocytes equal to monocytes, tissue macrophages, B cells, granulocytes (eosinophils, basophils, neutrophils), NK cells and some T cells. Fc receptors are moreover present on mast cells, follicular dendritic cells, epithelial cells, endothelial cells, hepatocytes and langerhans cells amongst others. There are a selection of types of Fc receptors (FcR) which can be categorised based on the antibody that they acknowledge. There are individuals who have good affinity for the Fc space of monomeric IgG antibody and are known as Fc-Gamma receptors, individuals who bind to IgA antibody are known as Fc-alpha receptors and individuals who bind IgE antibody are known as Fc-epsilon receptors.
Non explicit Fc receptors staining in assays equal to IHC, ICC, Immunofluorescence (IF) and motion cytometry might find yourself from the binding of the Fc space of the primary and/or secondary antibody or immunoglobulin isotypes to the Fc receptors of the cells. Eliminating Fc receptor staining is fascinating in IHC, ICC, IF and Circulation cytometry assays for the goal of right data evaluation.
For IHC, ICC, IF testing: The binding of the Fc space of main and/or secondary antibody and immunoglobulin
isotypes to the Fc receptors present on cells/tissues give rise to non-specific Fc receptor staining. Specimens rich in Fc receptors embrace spleen, lymphoid tissues, lymphomas, tonsil, lymph nodes, bone marrow, blood smears and liver. Others embrace tissues and cell preps meant for staining for CD markers; Immunoglobulins (Igs), Kappa and Lambda and tumor cell traces that specific Fc receptors. To steer clear of Fc receptor staining; tissue sections and cell preparations are incubated with Innovex Fc Blocker for a short interval earlier to utility of main antibody or immunoglobulin isotypes (See instruction a part of this data sheet).
For Circulation cytometry assays: Fc receptors are present on leukocytes (white blood cells), many cell traces along with completely different
cell varieties in every human and animal cells/tissues. Fc receptors affords rise to non-specific Fc receptor-staining in motion
cytometry assays by binding of Fc space of antibodies and isotype controls to Fc receptors of the cells. Non-specific Fc receptors staining is perhaps eradicated by incubating cells with INNOVEX Fc Receptor Blocker for a variety of Circulation cytometry assays equal to CD phenotyping, leukemia typing and cell line analysis. (See instruction a part of this data sheet).
