Lipid Peroxidation (MDA) Assay Kit

Product overview

Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) provides a helpful software program for delicate detection of malondialdehyde (MDA).

Inside the lipid peroxidation assay protocol, the MDA inside the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct might be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/successfully colorimetrically and 0.1 nmol/successfully fluorometrically.

The MDA assay will also be refered to as a TBARS assay.

Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid peroxidation is a extensively identified occasion of oxidative hurt in cell membranes, lipoproteins, and totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl cholesterol can occur in a number of reactions. They’re typically triggered by i) free radical species equivalent to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low cost of hydrogen peroxide or ii) non-radical species equivalent to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are very important toxic byproducts of lipid peroxidation. So, the measurement of the portions of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a critical product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and various organs beneath oxidative stress circumstances. The reality is, MDA is in a lot of conditions most likely essentially the most ample specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and loads of totally different biomolecules.

Bioquochem’s LPO assay tools measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Due to this, this response offers a diindolylalkane (chromophore) with most absorbance inside the space of 580-620 nm.

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.




Lipid peroxidation assay protocol summary:
– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For better sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.

Chinese language language protocol on the market. See protocols half underneath.

For an alternate MDA assay, with out the heating steps required inside the TBARS assay, try MDA assay ab233471.

  • Notes

    Lipid peroxidation refers again to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (sometimes in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is essential to guage oxidative stress. Lipid peroxidation varieties reactive aldehydes equivalent to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are generally used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

    Related merchandise

    Evaluation the oxidative stress marker and assay data, or the overall metabolism assay data to check further assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and as well as how one can assay metabolic function in dwell cells using your plate reader.

    Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.

    Lipid Peroxidation biovision
    Lipid Peroxidation biovision

    How totally different researchers have used Lipid Peroxidation Assay Bundle ab118970

    The MDA/TBARs assay tools has been utilized in publications in a variety of sample kinds, along with:
    – Human: serum1, hippocampal principal cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
    – Mouse: neuronal cell lysates5, coronary coronary heart tissue extract6, plasma7, cell extracts8
    – Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
    – Pig: serum12

    References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019

  • Platform

    Microplate reader

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