Get hold of fixed retroviral transductions
A severe advantage of retroviral gene-delivery strategies is the facility to control the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your purpose cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.
The best way it really works
The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (supplied) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the same old curve.
RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, can be exactly quantified using this gear.
Description
abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and can be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the gear ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally appropriate with most qPCR gadgets.
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Virus Titration Kits
Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.
Advantages of abm’s titration kits:
- No RNA purification — saves time and eliminates inaccuracy.
- Ready-to-use reagent mix — reduces variability.
- qRT-PCR in a single step — additional delicate and proper than completely different methods.
- No NTC amplification — Distinctive primer design totally eliminates non-specific amplification in NTCs.
We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which enable quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.
he Retro-X qRT-PCR Titration Gear provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.
Quick willpower of retroviral titer
Whereas typical titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the similar day.
qPCR Retrovirus Titer Kit |
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| E36G949 | EnoGene | 100 rxn | EUR 242.86 |
qPCR Retrovirus Titer Kit |
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| MBS8580089-100Reactions | MyBiosource | 100Reactions | EUR 340 |
qPCR Retrovirus Titer Kit |
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| MBS8580089-5x100Reactions | MyBiosource | 5x100Reactions | EUR 1540 |
AAV3 Titration ELISA Kit |
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| AAV-A003 | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
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Description: AAV3 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV3 capsid titration in AAV gene therapy product preparation processing. It contains AAV3 empty capsids and a pair of antibodies against AAV3. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV3 capsid titration. |
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AAV3 Titration ELISA Kit |
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| AAV-A003-96tests | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
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Description: AAV3 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV3 capsid titration in AAV gene therapy product preparation processing. It contains AAV3 empty capsids and a pair of antibodies against AAV3. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV3 capsid titration. |
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AAV5 Titration ELISA Kit |
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| AAV-A005 | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
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Description: AAV5 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration. |
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AAV5 Titration ELISA Kit |
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| AAV-A005-96tests | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
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Description: AAV5 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration. |
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AAV8 Titration ELISA Kit |
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| DEIAAV8 | Creative Diagnostics | 96T | EUR 624 |
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Description: cell culture supernatants, purified virus preparations |
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AAV9 Titration ELISA Kit |
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| DEIAAV9 | Creative Diagnostics | 96T | EUR 624 |
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Description: cell culture supernatants, purified virus preparations |
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M13 Phage Titration ELISA Kit |
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| 55R-PRPHAGE | Fitzgerald | 96 tests | EUR 699 |
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Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles |
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AAV5 Titration ELISA Kit (Fast) |
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| AAV-A005H | ACROBIOSYSTEMS | 96tests | EUR 719.4 |
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Description: AAV5 Titration ELISA Kit(Fast) is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration. |
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M13 Phage Titration ELISA Kit |
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| MBS539756-5x96Tests | MyBiosource | 5x96Tests | EUR 6375 |
M13 Phage Titration ELISA Kit |
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| MBS539756-96Tests | MyBiosource | 96Tests | EUR 1375 |
Retrovirus qPCR Quantification Kit |
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| K1472-100 | Biovision | 100 Rxns | EUR 381.6 |
Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
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| 80155-G | Alpha Diagnostics | 1 kit | EUR 781.2 |
Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
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| 80155-M | Alpha Diagnostics | 1 kit | EUR 781.2 |