ATpase assay

Key Choices:

Non-radioactive, homogeneous assays
• Specific assays
• Useful: minimal sample preparation; fast protocols (1-2 hours )
• Worth environment friendly: 100 assays;
• Extreme Throughput Screening applicable
• Validated: using mammalian tissues, cells, natural fluids

BioVision affords full in vitro bioassays that are utilized by a whole lot of worldwide scientists in fairly a couple of sickness related areas like most cancers, diabetes, weight issues, coronary heart issues, liver sickness, kidney sickness, Alzheimer’s sickness and infectious diseases, and so forth., along with totally different evaluation areas equal to metabolism, neuroscience, epigenetics, oxidative stress, stem cells, protein extraction and sample preparation, and so forth. Our high-quality bioassays provide specificity, sensitivity, reproducibility and however fairly priced. Our mission is to ship revolutionary bioassays based on forefront evaluation to meet your scientific needs. BioVision’s bioassay kits may be utilized with a couple of of the most popular and customary evaluation instrumentation (i.e. microplate reader, motion cytometer, and microscopes).

 

Inorganic ions play fairly a couple of physiological roles in nature. Cations and anions participate in neurological transmission, muscle contraction, intra-cellular water transport, acid-base stability in blood, bone formation, vitality swap and storage. Furthermore, they carry out as cofactors or could also be found as moieties inside
totally different important biomolecules. BioVision has a terrific portfolio of assays measuring inorganic ions along with assays for sodium, calcium, phosphate and loads of others.

 

BioVision’s intensive microplate-based assay portfolio affords fairly a couple of merchandise that may be utilized for the detection/quantification of enzymatic train, quantification of explicit chemical analytes in natural samples and for
high-throughput inhibitor screening. Our merchandise profit from mixtures of proprietary enzymes and chemical probes, fastidiously engineered and characterised for the detection of associated targets. Our kits have been examined and validated using precise natural samples (equal to blood, serum, plasma, urine, saliva, cell and tissue lysates and foodstuffs), ensuring that analytes are detectable in real-world natural matrices.

Sample utility is possibly an essential challenge when deciding on the proper product to your evaluation needs. This will likely resolve the necessary assay sensitivity and subsequently the favored detection method. Our hottest merchandise profit from safe chromophores and fluorophores that current sturdy alerts over time. PicoProbe, our proprietary fluorophore, is no doubt some of the delicate probes obtainable out there. It’ll probably detect as little as 20 pmoles of analyte per analyzed sample correctly.

Metabolism performs a key operate in different areas equal to physiology, cell biology, and medicines. Evaluation in metabolism is dramatically elevated by the conclusion that many frequent human diseases equal to most cancers, diabetes, weight issues, hypoglycemia, hypolipidemia, phenylketonuria, neurodegeneration, and so forth. are ensuing from perturbations in metabolic pathways. Understanding the metabolic variations and attainable outliers is thus an essential aspect of disease-oriented evaluation. Then once more, reactive oxygen species (ROS), produced endogenously on account of standard cellular metabolic capabilities or from exterior sources, poses a serious menace to cells since publicity to ROS could find yourself in severedamage to DNA, protein, and lipids. Determining explicit varieties of oxidative hurt is usually the focal point of curiosity  in a number of varieties of cancers and different neurological diseases. Antioxidant defenses to cut back fluctuations in ROS are essential to cell survival and dictate approaches to sickness treatments to chop again menace of most cancers or age-related purposeful deterioration.

 

Nonetheless, natural processes are larger characterised by discovering out cellular interaction ensuing from exogenous parts. Preclinical and Scientific Evaluation rely upon assays that look at completely totally different aspects of cellular conduct (improvement by the use of the cell cycle, migration, invasion) and measure the content material materials of key establishing blocks inside the cell are usually used to look at the properly being of cells in custom and their response to different stimuli (migration inducers, cell cycle arrest drugs, ldl ldl cholesterol transport inhibitors and so forth.).
BioVision is proud to produce the broadest Metabolism Assay portfolio ranging from the detection of enzymatic train, metabolites equal to carbohydrates, lipids, amino acids, inorganic ions, serum elements, coenzymes & cofactors to oxidative stress, glycolysis pathway, and intermediary metabolism. Moreover, we offer a good assortment of assay kits, antioxidants, inhibitors/activators of key ROS avid gamers and a growing assortment of assays to test some elementary processes governing cellular biology.

 

ATPase Activity Assay Kit (Colorimetric)
K417-100	Biovision	each	EUR 516

QuantiChrom™ ATPase/GTPase Assay Kit
DATG-200	BioAssay Systems	200	EUR 389
Description: For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. Key Features: High sensitivity. Detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm. Samples: ATPase and GTPase. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.007 U/L.

E. coli DNA Helicase ATPase assay Kit Plus-100
DNAB100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500
DNAB500KE	ProFoldin	500 assays	EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-100
DNAB100KS	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. aureus DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500
DNAB500KS	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. aureus DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

E. coli gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-100
DNAB100KH	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x H. influenzae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-100
DNAB100KN	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. pneumoniae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-100
DNAB100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-500
DNAB500KH	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x H. influenzae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-500
DNAB500KN	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. pneumoniae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-500
DNAB500KP	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x P. aeruginosa DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KS	ProFoldin	100 assays	EUR 423.72
Description: This product includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

E. coli Topo IV ATPase Assay Kit Plus (enzyme included)
T4A-100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x E. coli Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of E. coli Topo IV ATPase activity in a 384-well assay format.

P. aeruginosa Gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reaction buffer, DNA, P. aeruginosa gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

S. pneumoniae Topo IV ATPase Assay Kit Plus (enzyme included)
T4A-100KN	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. pneumoniae Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. pneumoniae Topo IV ATPase activity in a 384-well assay format.

S. aureus Topoisomerase IV ATPase Assay Kit Plus (enzyme included)
T4A-100KS	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. aureus Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. aureus Topo IV ATPase activity in a 384-well assay format.

P. aeruginosa Topoisomerase IV ATPase Assay Kit Plus (enzyme included)
T4A-100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x P. aeruginosa Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of P. aeruginosa Topo IV ATPase activity in a 384-well assay format.

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (RPE Conjugate)
33239-05151	AssayPro	150 ug	EUR 513.6

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (APC Conjugate)
33239-05161	AssayPro	150 ug	EUR 513.6

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (FITC Conjugate)
33239-05141	AssayPro	150 ug	EUR 513.6

Potassium-Transporting ATPase (H/K-ATPase) AssayLite Antibody (PerCP Conjugate)
33239-05171	AssayPro	150 ug	EUR 565.2

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (RPE Conjugate)
33404-05151	AssayPro	150 ug	EUR 513.6

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (APC Conjugate)
33404-05161	AssayPro	150 ug	EUR 513.6

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (FITC Conjugate)
33404-05141	AssayPro	150 ug	EUR 513.6

Human ATPase Inhibitory Factor 1/IF1 AssayLite Antibody (PerCP Conjugate)
33404-05171	AssayPro	150 ug	EUR 565.2

ATPase, Na+/K+ Transporting (ATPase) Antibody
abx011699-100ug	Abbexa	100 ug	EUR 510

Rat Na+/K+ ATPase (Na/K ATPase) ELISA kit
E02N0538-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Na+/K+ ATPase (Na/K ATPase) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.