Molecular Detection of Coxiella Burnetii In Brazilian Artisanal Cheese

Background: World publications on Q fever have elevated after the 2007 epidemic within the Netherlands. Nonetheless, the epidemiology of Q fever/coxiellosis in Brazil remains to be poorly understood. Accordingly, there have been few research investigating the presence of Coxiella burnetii in dairy merchandise all over the world, particularly in Brazil, the place consumption of contemporary cheese made out of raw-milk may be very excessive.

Goal: This research was a random survey to evaluate the prevalence of C. burnetii by PCR in conventional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk.

Strategies: DNA extracted from 53 cheese samples have been analyzed by nested PCR with C. burnetii-specific primers and the merchandise confirmed by DNA sequencing.

Outcomes: Out of the 53 cheese samples 5 (9.43%) have been C. burnetii DNA-positive, every coming from one of many respective randomly chosen manufacturing agroindustries. Primarily based on our outcomes, it’s estimated that 1.62 tons/day of ready-to-eat cheese made out of raw-milk from a complete of 16.2 tons produced every day within the research area are contaminated with C. burnetii.

Conclusion: To our data, that is the primary report of extremely heat-resistant zoonotic pathogen in raw-milk Brazilian artisanal cheese. This meals security hazard has been fully uncared for in ready-to-eat raw-milk Brazilian artisanal cheese and will suggest potential threats to shoppers, since C. burnetii survives in artisanal cheese submitted to lengthy ripening durations.

Thus, this work established random and consultant baseline prevalence of C. burnetii on this meals product in Brazil. Additional epidemiological research, monitoring developments and setting management targets are warranted. Lastly, these outcomes level out the significance of together with C. burnetii in animal and public well being surveillance applications.

Key phrases: Brazil; Coxiella burnetii; Meals security; Minas artisanal cheese; Zoonosis.

Clostridium Botulinum Toxoid E Control Peptide (aa 2-17)
VAng-Lsx2761-500g	Creative Biolabs	500 µg	EUR 2545.2
Description: Clostridium Botulinum E Toxoid, Peptide.

NATtrol Clostridium difficile NAP1, External Run Control, Low (6 X 1 mL)
NATCDI(NAP1)-ERCL	Zeptometrix	6 X 1 mL	EUR 438

NATtrol Clostridium difficile NAP1, External Run Control, Medium (6 X 1 mL)
NATCDI(NAP1)-ERCM	Zeptometrix	6 X 1 mL	EUR 557

Core Panel Multi-Tumor control slides, set of 5
TS900	Innovex	Set of 5	EUR 145
Description: Core Panel Multi-Tumor control slides, set of 5

Core Panel Multi-Tumor control slides, set of 25
TS900-25	Innovex	Set of 25	EUR 425
Description: Core Panel Multi-Tumor control slides, set of 25

Breast Panel Multi-Tumor control slides, set of 5
TS901	Innovex	Set of 5	EUR 145
Description: Breast Panel Multi-Tumor control slides, set of 5

Breast Panel Multi-Tumor control slides, set of 25
TS901-25	Innovex	Set of 25	EUR 395
Description: Breast Panel Multi-Tumor control slides, set of 25

Goat IgG (-ve control for flow cytometry) (isotype control)
20011-100	Alpha Diagnostics	100 test	EUR 123.6

BSA Control for Age-BSA
2221-BSA	Biovision	each	EUR 210

BSA Control for AGE-BSA
35R-AA007	Fitzgerald	10 mg	EUR 268.8
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Flow Control Fitting for Flypad
FLY1254	Scientific Laboratory Supplies	EACH	EUR 101.84

Control for ER, 4 cases (1.5mm)
ERC081	Pantomics	1	EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for PR, 3 cases (1.5mm)
PRC061	Pantomics	1	EUR 102
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

Flow Control Fitting for Blowgun
FLY1252	Scientific Laboratory Supplies	EACH	EUR 80.95

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-2	Alpha Diagnostics	100 ug	EUR 270

Control for DOG1, 3 cases (1.5mm)
DOG1061	Pantomics	1	EUR 102
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for HER2, 4 cases (1.5mm)
HRC081	Pantomics	1	EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Control for Ckit, 6 samples (1.5mm)
CKIT061	Pantomics	1	EUR 102
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

SLLK, Control Peptide for TSP1 Inhibitor
HY-P0301	MedChemExpress	1mg	EUR 159.6

Control/Blocking peptide for Human WNT5a
WNT511-P	Alpha Diagnostics	100 ug	EUR 196.8

Rec. Human CD21 protein control for WB
CD21-C	Alpha Diagnostics	100 ul	EUR 343.2

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-100	ScyTek Laboratories	100 Slides	EUR 847.2

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-25	ScyTek Laboratories	25 Slides	EUR 278.4

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-5	ScyTek Laboratories	5 Slides	EUR 117.6

Thiostatin Rat protein, control for western
TSTN11-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse IgG (-ve control for flow cytometry)
20008-1-100	Alpha Diagnostics	100 tests	EUR 123.6

Rabbit IgG (-ve control for flow cytometry)
20009-1-100	Alpha Diagnostics	100 tests	EUR 123.6

NATtrol Respiratory Panel 2 (RP2) Controls (Ea)
NATRPC2-BIO	Zeptometrix	Ea	EUR 550.46
Description: Please contact Gentaur in order to receive the datasheet of the product.

Control Sections for FD NeuroSilver™ Kit
PCS101	FD Neurotechnologies	each	EUR 69.6

Purified, Human S100 protein control for WB
S1002-C	Alpha Diagnostics	100 ul	EUR 343.2

Control/Blocking peptide for Human WNT-1
WNT111-P	Alpha Diagnostics	100 ug	EUR 548.4

Negative Control Antibody for Mouse Monoclonals
V8785-100UG	NSJ Bioreagents	100ug	EUR 349.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

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V8785-20UG	NSJ Bioreagents	20ug	EUR 153.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

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V8785SAF-100UG	NSJ Bioreagents	100ug	EUR 349.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

SLLK, Control Peptide for TSP1 Inhibitor(TFA)
HY-P0301A	MedChemExpress	5mg	EUR 456

Purified, Bovine S100 protein control for WB
S1001-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat MEH Antibody
MEHCON	Detroit R&D	100 uL	EUR 150

Human Pancreatic Amylase protein control for Western blot
PAMY13-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Apolipoprotein B protein control for WB
APOB21-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat CYP2C Antibody
P2CCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP2D Antibody
P2DCON	Detroit R&D	100 uL	EUR 150

NATtrol Clostridium difficile Verification Panel (6 X 1 mL)
NATCDIVP-C	Zeptometrix	6 X 1 mL	EUR 586

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P2B1PTCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP3A2 Antibody
P3A2PTCON	Detroit R&D	100 uL	EUR 150

Haptoglobin Human Plasma protein control for WB
HGLB18-C	Alpha Diagnostics	100 ul	EUR 343.2

Control/Blocking peptide for Mouse Vimentin (Vim)
VIM11-C	Alpha Diagnostics	100 ug	EUR 196.8

NATtrol Respiratory Panel 2.1 (RP2.1) Controls (12 x 0.3mL)
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Mouse IgG Isotype control (purified) validated for
NG903	Innovex	7 ml	EUR 195
Description: Mouse IgG Isotype control (purified) validated for IHC; Ready-To-Use

Mouse IgG Isotype control (purified) validated for
NG903C	Innovex	1 ml	EUR 275
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Mouse IgM Isotype control (purified) validated for
NG906	Innovex	7 ml	EUR 265
Description: Mouse IgM Isotype control (purified) validated for IHC; Ready-To-Use

Mouse IgM Isotype control (purified) validated for
NG906C	Innovex	1 ml	EUR 295
Description: Mouse IgM Isotype control (purified) validated for IHC; Concentrate

Positive Control for Anti-Human CYP3A Antibody
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Eppendorf Centrifuge 5430R Knob Control Refrigerated- IVD Only
E5428000465	Scientific Laboratory Supplies	EACH	EUR 8115.6

Control/Blocking peptide for Ephrin-B2 antibody
NIVE2B-C	Alpha Diagnostics	100 ug	EUR 196.8

Positive Control for Anti-Human CYP1B1 Antibody
PH1B1CON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP2E1 Antibody
P2E1PTCON	Detroit R&D	100 uL	EUR 150

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Chicken Egg Ovalbumin protein control for Western
OVA11-C	Alpha Diagnostics	100 ul	EUR 343.2

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CP17-C	Alpha Diagnostics	100 ul	EUR 343.2

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Control-slides-	Innovex	Set of 5	EUR 119
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Positive control tissue section for each individua
Control-Slides-for-each-antibody	Innovex	Set of 25	EUR 355
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Control sections for FD NeuroApop™ Kit (frozen)
PCS102-	FD Neurotechnologies	each	EUR 87.6

Control sections for FD NeuroApop™ Kit (frozen)
PCS102-2	FD Neurotechnologies	each	EUR 80.54
Description: Best quality products

Human Native Retinol binding protein (RBP) control for Western
RBP41-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse Ceruloplasmin protein control for western blot
CP16-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified Flagellin (salmonella) protein control for WB
FLGN17-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Hemopexin purified protein control for Western
HPEX11-C	Alpha Diagnostics	100 ul	EUR 343.2

Hemopexin Mouse, purified protein control for Western
HPEX16-C	Alpha Diagnostics	100 ul	EUR 343.2

Q-VE-OPh (Negative Control for Q-VD-OPh)
GW8990-1	Glentham Life Sciences	1	EUR 228.1

Lectin binding native protein (positive control for SNA lectin)
LBP16-N-1	Alpha Diagnostics	1 ml	EUR 270

Human Apolipoprotein C-I protein control for WB
APOC11-C	Alpha Diagnostics	100 ul	EUR 343.2

Control sections for FD NeuroApop™ Kit (paraffin)
PCS102-1	FD Neurotechnologies	each	EUR 80.44
Description: Best quality products

Purified Human Survivin protein for WB +ve control
SURV11-C	Alpha Diagnostics	100 ul	EUR 343.2

Lectin binding native protein (negative control for all lectins)
LNP15-N-1	Alpha Diagnostics	1 ml	EUR 270

Human Apolipoprotein C-II protein control for WB
APOC22-C	Alpha Diagnostics	100 ul	EUR 343.2

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK21-C	Alpha Diagnostics	100 ul	EUR 343.2

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK22-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat CHP2B1/2 Antibody
P2B12PTCON	Detroit R&D	100 uL	EUR 150

Bovine Adenosine deaminase protein control for Western
ADA11-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for anti-GST p-forms: rat P
GSTCON3	Detroit R&D	100 uL	EUR 150

Human Apolipoprotein C-III protein control for WB
APOC32-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified human Catalase (CATL) protein control for WB
CATL11-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat CYP1A1/1A2 Antibody
P1ACON	Detroit R&D	100 uL	EUR 150

Purified bovine Lactoferrin protein control for Western
LTF13-C	Alpha Diagnostics	100 ul	EUR 343.2

Rat neurofascin (Nfasc)-control Control/blocking peptide
AB-23249-CP	Alpha Diagnostics	100ug	EUR 196.8

Chicken Egg white proteins total control for Western
CEGW12-C	Alpha Diagnostics	100 ul	EUR 343.2

Rabbit Beta Actin (muscle) protein control for Western
ACTB15-C	Alpha Diagnostics	100 ul	EUR 388.8

Human plasma Apolipoprotein E protein control for WB
APOE13-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Plasma Apolipoprotein J protein control for WB
APOJ13-C	Alpha Diagnostics	100 ul	EUR 343.2

Chicken Egg yellow proteins total control for Western
CEYP12-C	Alpha Diagnostics	100 ul	EUR 343.2

Chicken Serum Albumin protein control for Western blot
ALBC15-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Salivary Amylase protein positive control for WB
SAMY16-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified, recombinant Human TACE control protein for WB
TACE11-C	Alpha Diagnostics	100 ul	EUR 343.2

pMD18- Control
PVT10563	Lifescience Market	2 ug	EUR 319.2

pMD19- Control
PVT10564	Lifescience Market	2 ug	EUR 319.2

Purified Cholera Toxin protein control for Western Blot
CTOX11-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Prothrombin (Factor II) protein control for Western
PRTN11-C	Alpha Diagnostics	100 ul	EUR 343.2