Molecular Detection of Coxiella Burnetii In Brazilian Artisanal Cheese

Background: World publications on Q fever have elevated after the 2007 epidemic within the Netherlands. Nonetheless, the epidemiology of Q fever/coxiellosis in Brazil remains to be poorly understood. Accordingly, there have been few research investigating the presence of Coxiella burnetii in dairy merchandise all over the world, particularly in Brazil, the place consumption of contemporary cheese made out of raw-milk may be very excessive.

Goal: This research was a random survey to evaluate the prevalence of C. burnetii by PCR in conventional Minas artisanal cheese from the Serro microregion, Brazil, which is manufactured from bovine raw-milk.

Strategies: DNA extracted from 53 cheese samples have been analyzed by nested PCR with C. burnetii-specific primers and the merchandise confirmed by DNA sequencing.

Outcomes: Out of the 53 cheese samples 5 (9.43%) have been C. burnetii DNA-positive, every coming from one of many respective randomly chosen manufacturing agroindustries. Primarily based on our outcomes, it’s estimated that 1.62 tons/day of ready-to-eat cheese made out of raw-milk from a complete of 16.2 tons produced every day within the research area are contaminated with C. burnetii.

Conclusion: To our data, that is the primary report of extremely heat-resistant zoonotic pathogen in raw-milk Brazilian artisanal cheese. This meals security hazard has been fully uncared for in ready-to-eat raw-milk Brazilian artisanal cheese and will suggest potential threats to shoppers, since C. burnetii survives in artisanal cheese submitted to lengthy ripening durations.

Thus, this work established random and consultant baseline prevalence of C. burnetii on this meals product in Brazil. Additional epidemiological research, monitoring developments and setting management targets are warranted. Lastly, these outcomes level out the significance of together with C. burnetii in animal and public well being surveillance applications.

Key phrases: Brazil; Coxiella burnetii; Meals security; Minas artisanal cheese; Zoonosis.

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