Glycolaldehyde-Derived High-Molecular-Weight Advanced Glycation End-Products Induce Cardiac Dysfunction through Structural and Functional Remodeling of Cardiomyocytes

Glycolaldehyde-Derived High-Molecular-Weight Advanced Glycation End-Products Induce Cardiac Dysfunction through Structural and Functional Remodeling of Cardiomyocytes

Background/goals: Excessive-molecular-weight superior glycation end-products (HMW-AGEs) are abundantly current in our Western weight loss plan. There’s rising proof reporting that HMW-AGEs contribute to the event of cardiovascular dysfunction in vivo, subsequent to the well-known low-molecular-weight AGEs. The purpose of our research is to evaluate the ultrastructure and performance of cardiomyocytes after power publicity to HMW-AGEs. A greater understanding of underlying mechanisms is crucial to create new alternatives for additional analysis on the particular function of HMW-AGEs within the improvement and development of cardiovascular ailments.
Strategies: Grownup male rats have been randomly assigned to every day intraperitoneal injection for six weeks with both HMW-AGEs (20 mg/kg/day) or a management answer. Hemodynamic measurements have been carried out at sacrifice. Single cardiomyocytes from the left ventricle have been obtained by enzymatic dissociation via retrograde perfusion of the aorta. Unloaded cell shortening, time to peak and time to 50% leisure have been measured throughout subject stimulation and normalized to diastolic size. L-type Ca2+ present density (ICaL) and steady-state inactivation of ICaL have been measured throughout whole-cell ruptured patch clamp. Myofilament useful properties have been measured in membrane-permeabilized cardiomyocytes. Ultrastructural examination of cardiac tissue was carried out utilizing electron microscopy.
Outcomes: Rats injected with HMW-AGEs displayed in vivo cardiac dysfunction, characterised by important adjustments in left ventricular peak price stress rise and decline accompanied with an elevated coronary heart mass. Single cardiomyocytes remoted from the left ventricle revealed concentric hypertrophy, indicated by the rise in mobile width. Unloaded fractional cell shortening was considerably diminished in cells derived from the HMW-AGEs group and was related to slower kinetics. Peak L-type Ca2+ present density was considerably decreased within the HMW-AGEs group. L-type Ca2+ channel availability was considerably shifted in direction of extra unfavourable potentials after HMW-AGEs injection. The affect of HMW-AGEs on myofilament operate was measured in membrane-permeabilized cardiomyocytes exhibiting a discount in passive drive, maximal Ca2+ activated drive and price of drive improvement. Ultrastructural examination of cardiac tissue demonstrated antagonistic structural transforming in the HMW-AGEs group characterised by a disruption of the cyto-architecture, a decreased mitochondrial density and altered mitochondrial operate.
Conclusion: Our knowledge point out that HMW-AGEs induce structural and useful mobile transforming through a distinct working mechanism because the well-known LMW-AGEs. Outcomes of our analysis open the door for brand spanking new methods concentrating on HMW-AGEs to enhance cardiac final result.

New Mammalian Goal of Rapamycin (mTOR) Modulators Derived from Pure Product Databases and Marine Extracts by Utilizing Molecular Docking Strategies.

Mammalian goal of rapamycin (mTOR) is a PI3K-related serine/threonine protein kinase that features as a grasp regulator of mobile development and metabolism, in response to nutrient and hormonal stimuli. mTOR features in two distinct complexes-mTORC1 is delicate to rapamycin, whereas, mTORC2 is insensitive to this drug. Deregulation of mTOR’s enzymatic exercise has roles in most cancers, weight problems, and getting older. Rapamycin and its chemical derivatives are the one medicine that inhibit the hyperactivity of mTOR, however quite a few negative effects have been described on account of its therapeutic use. The aim of this research was to determine new compounds of pure origin that may result in medicine with fewer negative effects.
We’ve used computational strategies (molecular docking and calculated ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) parameters) which have enabled the collection of candidate compounds, derived from marine pure merchandise, SuperNatural II, and ZINC pure merchandise, for inhibitors concentrating on, each, the ATP and the rapamycin binding websites of mTOR. We’ve proven experimental proof of the inhibitory exercise of 11 chosen compounds towards mTOR. We’ve additionally found the inhibitory exercise of a brand new marine extract towards this enzyme. The outcomes have been mentioned regarding the necessity to determine new molecules for therapeutic use, particularly towards getting older, and with fewer negative effects.
Glycolaldehyde-Derived High-Molecular-Weight Advanced Glycation End-Products Induce Cardiac Dysfunction through Structural and Functional Remodeling of Cardiomyocytes
Glycolaldehyde-Derived High-Molecular-Weight Advanced Glycation End-Products Induce Cardiac Dysfunction through Structural and Functional Remodeling of Cardiomyocytes

Isolation and Molecular Identification of Bacteriocin-producing Enterococci with Broad Antibacterial Exercise from Conventional Dairy Merchandise in Kerman Province of Iran.

One of many crucial limitations to make use of of bacteriocins produced by lactic acid micro organism as an alternative choice to chemical antibiotics is the slender spectrum of their antibacterial exercise. The intention of current research was isolation and molecular identification of bacteriocin-producing enterococci with broad antibacterial spectrum.
Bacteriocin-producing micro organism have been remoted from native dairies in Kerman. Bacteriocins have been purified by ammonium sulfate technique and the results of them have been investigated on totally different strains of micro organism. Additionally, the results of pH and warmth on produced bacteriocins have been investigated. Excessive stage bacteriocin-producing isolates have been recognized based mostly on molecular exams. A complete of 15 strains of bacteriocin-producing Enterococcus have been remoted initially.

Urea, suitable for molecular biology

GE1210-1KG 1 kg
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Urea, suitable for molecular biology

GE1210-500G 500 g
EUR 76.8

Sucrose, GlenBiol, suitable for molecular biology

GC3201-1KG 1 kg
EUR 90

BCIP (Molecular Biology Grade)

CE108 250 mg
EUR 75.6

BCIP (Molecular Biology Grade)

CE109 1 g
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CHAPS (Molecular Biology Grade)

CE114 1 g
EUR 66

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 157.2

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 492

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
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Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 66

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
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DTT (Molecular Biology Grade)

CE131 5 g
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DTT (Molecular Biology Grade)

CE132 10 g
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DTT (Molecular Biology Grade)

CE133 25 g
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Glycine (Molecular Biology Grade)

CE158 1 kg
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Glycine (Molecular Biology Grade)

CE159 5 kg
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HEPES (Molecular Biology Grade)

CE171 100 g
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CE172 500 g
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CE173 1 kg
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Lysozyme (Molecular Biology Grade)

CE188 1 g
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Lysozyme (Molecular Biology Grade)

CE189 10 g
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NAD (Molecular Biology Grade)

CE196 1 g
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NAD (Molecular Biology Grade)

CE197 5 g
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NBT (Molecular Biology Grade)

CE209 1 g
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NBT (Molecular Biology Grade)

CE210 5 g
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Tris (Molecular Biology Grade)

CE237 500 g
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Tris (Molecular Biology Grade)

CE238 1 kg
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Tris (Molecular Biology Grade)

CE239 5 kg
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Tween20 (Molecular Biology Grade)

CE242 1 l
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Water (Molecular Biology Grade)

CE243 500 ml
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Water (Molecular Biology Grade)

CE244 1 l
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100mL Molecular Biology Grade

46-000-CI PK6
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500mL Molecular Biology Grade

46-000-CV PK6
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Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
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Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
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Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
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Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
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Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
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Formamide deionized (Molecular Biology Grade)

CE145 500 ml
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Formamide deionized (Molecular Biology Grade)

CE146 1 l
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Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
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Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
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Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
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Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
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Guanidine - Hydrochloride (Molecular Biology Grade)

CE160 100 g
EUR 93.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE161 250 g
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Guanidine - Hydrochloride (Molecular Biology Grade)

CE162 500 g
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Guanidine - Hydrochloride (Molecular Biology Grade)

CE163 1 kg
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Guanidine Thiocyanate (Molecular Biology Grade)

CE164 100 g
EUR 86.4

Guanidine Thiocyanate (Molecular Biology Grade)

CE165 500 g
EUR 192

Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
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MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 102

MOPS buffer (Molecular Biology Grade)

CE195 250 g
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Sodium chloride (Molecular Biology Grade)

CE205 500 g
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Sodium chloride (Molecular Biology Grade)

CE206 1 kg
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Sodium chloride (Molecular Biology Grade)

CE207 5 kg
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D(+)-Sucrose (Molecular Biology Grade)

CE224 500 g
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D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
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D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
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Tris - Hydrochloride (Molecular Biology Grade)

CE234 250 g
EUR 99.6

Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 144

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
EUR 223.2

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 67.2

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 79.2

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
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Description: Minimum order quantity: 1 unit of 4L

Tween 20, Molecular Biology Grade

T9100-010 100ml
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Tween 20, Molecular Biology Grade

T9100-050 500ml
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Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 160.8

1L Molecular Biology Grade Water

46-000-CM PK6
EUR 177.84

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 92.4

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 217.2

Crystalline

5-01010 10mg Ask for price

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE135 250 g
EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE136 500 g
EUR 86.4

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE137 1 kg
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EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE138 5 kg
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D(+)-Glucose waterfree (Molecular Biology Grade)

CE148 500 g
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D(+)-Glucose waterfree (Molecular Biology Grade)

CE149 1 kg
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D(+)-Glucose waterfree (Molecular Biology Grade)

CE150 5 kg
EUR 180

Yeast extract powder (Molecular Biology Grade)

CE169 500 g
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Hyaluronidase Grade I (Molecular Biology Grade)

CE174 1 g
EUR 232.8

Hyaluronidase Grade I (Molecular Biology Grade)

CE175 5 g
EUR 920.4

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 98.4

NADH - Disodium salt (Molecular Biology Grade)

CE198 1 g
EUR 91.2

NADH - Disodium salt (Molecular Biology Grade)

CE199 5 g
EUR 244.8

NADP - sodium salt (Molecular Biology Grade)

CE200 250 mg
EUR 92.4

NADP - sodium salt (Molecular Biology Grade)

CE201 1 g
EUR 190.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 70.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 126

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 374.4

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
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XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 208.8

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 612

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE100 50 g
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Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE101 100 g
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Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE102 250 g
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Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE103 500 g
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Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE104 1 kg
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Water, Ultra Pure, Sterile Molecular Biology Grade

18-193 100ml/Unit
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Water, Ultra Pure, Sterile Molecular Biology Grade

18-194 500ml/Unit
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Water, Ultra Pure, Sterile Molecular Biology Grade

18-195 1000ml/Unit
EUR 308

Water, Ultra Pure, Sterile Molecular Biology Grade

18-196 4 x 1L/Unit
EUR 471

EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade)

CE141 500 ml
EUR 87.6

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE178 500 g
EUR 109.2

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE179 2.5 kg
EUR 301.2

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE180 500 g
EUR 104.4

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE181 2.5 kg
EUR 295.2

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE182 500 g
EUR 108

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE183 2.5 kg
EUR 301.2

LB-Medium - Powder according to Miller (Molecular Biology Grade)

CE184 2.5 kg
EUR 295.2

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 266.4
Description: Minimum order quantity: 1 unit of 100G

Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g

41028-25G 25G
EUR 130.8
Description: Minimum order quantity: 1 unit of 25G

Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g

41028-500G 500G
EUR 862.8
Description: Minimum order quantity: 1 unit of 500G

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 201.6
Description: Bioscience Mol Bio; Agarose

Bulk Beads Zr 0.1mm Triple-Pure Molecular Biology Grade 250g

HOM3048 250G
EUR 536.94

Bulk Beads Zr 0.5mm Triple-Pure Molecular Biology Grade 250g

HOM3050 250G
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Bulk Beads Zr 1.0mm Triple-Pure Molecular Biology Grade 250g

HOM3052 250G
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Bulk Beads Zr 1.5mm Triple-Pure Molecular Biology Grade 250g

HOM3054 250G
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Bulk Beads Zr 3.0mm Triple-Pure Molecular Biology Grade 300g

HOM3056 300G
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Phenol

20-abx184753
  • EUR 292.80
  • EUR 260.40
  • 1 kg
  • 500 g

Phenol

S-3030 1ML
EUR 38.76

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3669.6
Description: Bioscience Mol Bio; Agarose

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

Hettich Lid For Rotor Phenol Resistant

CEN0811 EACH
EUR 282.72

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 926.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2490
Description: Bioscience Mol Bio; Agarose

Crystalline [H-Trp-Gly-OH; MW: 261.2]

SP-55342-1 1 mg
EUR 169.2

2 Deoxyadenosine 5Triphosphate Disodium Crystalline dATP 250mg

27185004 EACH
EUR 355.68

Nonyl Phenol

AG037 1 mg
EUR 627.6

Water Phenol

abx082109-250ml 250 ml
EUR 226.8

Nonyl Phenol

AT037 1mg
EUR 1641.6

Phenol, 99%

GK6990-100G 100 g
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Phenol, 99%

GK6990-1KG 1 kg
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Phenol, 99%

GK6990-500G 500 g
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Phenol Red

GT9844-100G 100 g
EUR 132

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GT9844-250G 250 g
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Phenol Red

GT9844-25G 25 g
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Phenol Red

GT9844-5G 5 g
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Phenol red

PB0420 25g
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Phenol-d6

S-3035 1ML
EUR 38.76
Enterococcus faecium C-2 and Y-1 strains produced bacteriocins with the very best antibacterial impact. The bacteriocins have been secure in pH ranges from 2 to 12 and their antibacterial exercise was maintained after autoclave therapy. The utmost bactericidal impact was noticed towards Listeria monocytogenes and Pseudomonas aeruginosa. In conclusion, use of those bacteriocins as an alternative choice to chemical antibiotics is beneficial.

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